Protein Disulfide Isomerase from bovine liver
SIGMA/P3818 - ≥100 units/mg protein, lyophilized powder
Synonym: PDI
CAS Number: 37318-49-3
MDL Number: MFCD00163364
Product Type: Chemical
PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.
SDS-PAGE Protein Disulfide Isomerase from Bovine liver (Cat. No. P3818) was assessed on SDS-PAGE.
| composition | Protein, ~10% Lowry |
| form | lyophilized powder |
| InChI | 1S/C7H5Cl2NO5S/c8-4-2-5(9 |
| InChI key | DHUYKLYJBKXDBM-UHFFFAOYSA |
| mol wt | 107 kDa |
| Quality Level | 200  |
| SMILES string | [S](=O)(=O)(ON)c1c(cc(c(c |
| specific activity | ≥100 units/mg protein |
| storage temp. | −20°C |
| Application: | Protein Disulfide Isomerase from bovine liver has been used: • to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi • in the in vitro translation reaction for the generation of disulfide bonds • in insulin-disulfide reduction assay and peptide binding assay • as a positive control in thiol-disulfide oxidoreductase activity assay |
| Biochem/physiol Actions: | Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges. It aids protein refolding in vitro allowing recombinant proteins to achieve their native state. |
| Biochem/physiol Actions: | Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings. |
| General description: | Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucos |
| General description: | Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2. The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucos |
| Other Notes: | One unit cause a change in A650 of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C. |
| Packaging: | 0.1 mg in poly bottle |
| Packaging: | 250 μg in glass bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing potassium phosphate buffer salts and stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 5.3.4.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |