Anti-phospho-PKB (pThr308) antibody produced in rabbit
SIGMA/P3862 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-phospho-Akt
MDL Number: MFCD02686506
Product Type: Chemical
Western Blotting Whole extract of PDGF-treated Mouse fibroblast NIH3T3 cells were separated on SDS-PAGE and probed with Anti-phospho-PKB (pThr308) antibody produced in Rabbit (Cat. No. P3862). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:1,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 56 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr308) |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H2O2-treated rat fibroblast Rat-1 cells | |
| UniProt accession no. | P31750  |
| Application: | Anti-phospho PKB (pThr308) may be used for detection and localization of phospho-PKB by immunoblotting. A minimum dilution of 1:1000 may be used for detection by immunoblotting in PDGF-treated mouse NIH3T3 cells and peroxide-treated Rat-1 fibroblast cell line. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr308 and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death Anti-phospho PKB (pThr308) recognises PKB phosphorylated at threonine 308 (56 kDa). |
| Immunogen: | synthetic phosphopeptide corresponding to the pThr308 region of human PKBα (amino acids 301-315). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |