Anti-Pyk2 antibody produced in rabbit
SIGMA/P3902 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CAK-β; Anti-Proline rich Kinase 2
MDL Number: MFCD01634069
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human cerebellum tissue sections were stained with 1:100 Anti-Pyk2 antibody produced in Rabbit (Cat. No. P3902) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of LPS-stimulated P388 Mouse monocyte-macrophage cells were separated on SDS-PAGE and probed with Anti-Pyk2 antibody produced in Rabbit (Cat. No. P3902). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 110-116 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections of human cerebellum. |
| immunoprecipitation (IP): 3-5 μg/mL using 150-200 μg of PC-12 rat pheochromocytoma RIPA lysate | |
| microarray: suitable | |
| western blot: 1:2,000 using a whole extract of LPS-stimulated P388 mouse monocyte-macrophage cells | |
| UniProt accession no. | Q14289  |
| Application: | Anti-Pyk2 antibody produced in rabbit has been used in: • immunocytochemistry • immunohistochemistry • western blotting |
| Biochem/physiol Actions: | Protein tyrosine kinases (PTKs) are critical components of the signalling pathways that control cell growth, differentiation, apoptosis, metabolism, cell cycle regulation and cytoskeletal function. Pyk2 has been detected in cell-cell contacts, at focal adhesion-like structures and podosomes, cytoplasmic perinuclear region, in association with actin filaments and diffusely distributed in the cytoplasm. Pyk2 phosphorylation is critical for its interaction with SH2-containing signalling molecules and their linkage to signalling pathways that regulate extracellular-signal-regu |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Pyk2 (proline-rich kinase 2) protein belongs to tyrosine protein kinase family and is primarily expressed in the central nervous system and in cells derived from hematopoietic lineages. Pyk2 is found in tissues and cells like mesenchymal, epithelial, endothelial cells, neonatal cardiomyocytes, osteoclasts and neuronal cells. |
| Immunogen: | Synthetic peptide corresponding to amino acid residues 991-1009 of human Pyk2, coupled to KLH. This sequence is highly conserved in rat and mouse (1 amino acid substitution). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |