Phospholipase C from Clostridium perfringens (C. welchii)
SIGMA/P4039 - Type XIV, lyophilized powder, ≥150 units/mg protein
Synonym: Lecithinase C; Lipophosphodiesterase I; PC-PLC; Phosphatidylcholine cholinephosphohydrolase
CAS Number: 9001-86-9
EC Number: 232-638-2
MDL Number: MFCD00131895
Product Type: Chemical
Degumming of feedstock oils by hydrolyisis of phospholipids can be catalyzed by phospholipase C. Treatment of
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| biological source | bacterial (Clostridium perfringens) |
| form | lyophilized powder |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | ≥150 units/mg protein |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| type | Type XIV |
| Application: | Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA. |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk |
| Packaging: | 125, 500 units in serum bottle |
| Physical form: | Lyophilized powder in buffered salts |
| Preparation Note: | Chromatographically purified |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥150 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 41106300 |