Phosphatase, Alkaline from Escherichia coli
SIGMA/P4069 - buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
Synonym: Orthophosphoric-
CAS Number: 9001-78-9
EC Number: 232-631-4
MDL Number: MFCD00131849
Product Type: Chemical
Unit Definition
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Approximately 3 DEA units equal one Glycine unit.
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Approximately 3 DEA units equal one Glycine unit.
| biological source | Escherichia coli |
| foreign activity | DNase, RNase, none detected |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | 20-50 units/mg protein (in glycine buffer) |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution. |
| Application: | Phosphatase, Alkaline from Escherichia coli has been used: • in fluorometric assay to determine its enzyme activity • in the dephosphorylating enzyme cocktail for dephosphorylation of peptides • in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity |
| Biochem/physiol Actions: | Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| Disclaimer: | Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C. |
| Physical form: | Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 20-50 units/mg protein (in glycine buffer) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |