Phosphatase, Alkaline from Escherichia coli
SIGMA/P4252 - ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Synonym: Orthophosphoric-
CAS Number: 9001-78-9
EC Number: 232-631-4
MDL Number: MFCD00131849
Product Type: Chemical
Unit Definition One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Approximately 3 DEA units equal one Glycine unit.
| biological source | Escherichia coli |
| form | ammonium sulfate suspension |
| Quality Level | 300  |
| specific activity | 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer) |
| storage temp. | 2-8°C |
| Analysis Note: | The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C. |
| Application: | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein. |
| Application: | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer. |
| Biochem/physiol Actions: | Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| Biochem/physiol Actions: | Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| Disclaimer: | Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C. |
| Packaging: | 100, 200, 1000 units in glass bottle |
| Physical form: | Suspension in 2.5 M (NH4)2SO4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |