Pepstatin A
SIGMA/P4265 - microbial, ≥75% (HPLC)
Synonym: Pepsin inhibitor
CAS Number: 26305-03-3
Empirical Formula (Hill Notation): C34H63N5O9
Molecular Weight: 685.89
EC Number: 247-600-0
MDL Number: MFCD00060740
Linear Formula: C34H63N5O9
Product Type: Chemical
| assay | ≥75% (HPLC) |
| biological source | microbial |
| form | powder |
| InChI | 1S/C34H63N5O9/c1-17(2)12- |
| InChI key | FAXGPCHRFPCXOO-LXTPJMTPSA |
| mp | 233 °C (dec.) (lit.) |
| Quality Level | 300  |
| SMILES string | CC(C)C[C@H](NC(=O)[C@H](C |
| solubility | ethanol: acetic acid (9:1): 1 mg/mL, clear to hazy, colorless |
| storage temp. | 2-8°C |
| Application: | Can be used in conjunction with E64-d and Leupeptin A to inhibit the degradation of autophagic cargo inside autophagosomes. For this application, the working concentration is typically between 1-10 μM. |
| Application: | Pepstatin A has been used: • as a component of synaptoneurosome buffer to homogenize the C57BL/6 striata • as a component of cyclin-dependent kinase 5 (Cdk5) lysis buffer • as an inhibitor to determine the chymotrypsin activity of California spiny lobster |
| Biochem/physiol Actions: | Pepstatin A is an inhibitor of acid proteases (aspartyl peptidases). It forms a 1:1 complex with proteases such as pepsin, renin, cathepsin D, bovine chymosin, and protease B (Aspergillus niger). The inhibitor is highly selective and does not inhibit thiol proteases, neutral proteases, or serine proteases. Solubilized γ-secretase and retroviral protease are also inhibited by Pepstatin A. It has been used to characterize proteases from several sources. |
| Biochem/physiol Actions: | Potent inhibitor of acid proteases, including pepsin, renin and cathepsin D and many microbial aspartic proteases. Effective concentration 1 μM. Stable for about 1 day. |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | 100, 250 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Preparation Note: | Pepstatin can be dissolved at 1 mg/ml in 10% (v/v) acetic acid in methanol (9:1 methanol:acetic acid). The inclusion of acetic acid may be necessary to dissolve this peptide in methanol or DMSO. It has been dissolved at 1 to 2 mg/ml in ethanol, but heat may be required for complete dissolution. Solutions of Pepstatin A can be heated as high as 60 °C without any decomposition of the peptide. Stock solutions at 1 mg/ml are stable at least a week at 4 °C. A 1 mM solution in methanol or DMSO is stable for months at -20 °C. If solutions become darker yellow, the reagent is hydrolyzing. A working concentration of 1 μM is stable for at least one day at room temperature. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥75% (HPLC) |
| mp | 233 °C (dec.) (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |