Platelet-Derived Growth Factor-BB human
SIGMA/P4306 - PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: PDGF-BB
MDL Number: MFCD00213023
Product Type: Chemical
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| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | 24.3 kDa |
| packaging | pkg of 10 μg |
| potency | 1.0-3.0 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01127  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells. |
| Application: | Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum. |
| Biochem/physiol Actions: | Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding. |
| General description: | Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. |
| Other Notes: | Dimer of the B chain of human PDGF. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |