Papain Agarose from papaya latex
SIGMA/P4406 - lyophilized powder, 90-150 units/mL packed gel
MDL Number: MFCD00131791
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4406-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4406-50UN
| biological source | papaya (latex) |
| extent of labeling | 3,000-5,000 units per g agarose (at pH7.0 at 30 °C.) |
| form | lyophilized powder |
| matrix | beaded agarose |
| mol wt | 21 kDa |
| Quality Level | 200  |
| specific activity | 90-150 units/mL packed gel |
| storage temp. | −20°C |
| Application: | Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments. |
| Application: | Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. |
| Biochem/physiol Actions: | A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. |
| Biochem/physiol Actions: | Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. . The pH optimum is 6.0-7.0 . Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below. |
| Packaging: | 25, 50, 100 units in glass bottle |
| Physical form: | Lyophilized powder, stabilized with lactose |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 90-150 units/mL packed gel |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |