Phosphatase, Alkaline from porcine kidney
SIGMA/P4439 - lyophilized powder, ≥100 DEA units/mg protein
Synonym: Orthophosphoric-
CAS Number: 9001-78-9
EC Number: 232-631-4
MDL Number: MFCD00131849
Product Type: Chemical
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| composition | Protein, ≥50% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥100 DEA units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling. |
| Biochem/physiol Actions: | Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C. |
| Packaging: | 100 units in serum bottle |
| Packaging: | Package size based on DEA units |
| Physical form: | Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 DEA units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |