β-Lactamase from Enterobacter cloacae
SIGMA/P4524 - Type IV, lyophilized powder, 0.2-0.6 units/mg protein (using benzylpenicillin)
Synonym: β-Lactamase I; β-Lactamase II; Cephalosporinase; Penicillin amido-β-lactam hydrolase; Penicillinase from Enterobacter cloacae
CAS Number: 9073-60-3
MDL Number: MFCD00131819
Product Type: Chemical
Unit Definition One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P4524-100UN
| application(s) | pharmaceutical |
| biological source | bacterial ( |
| composition | Protein, ~60% |
| form | lyophilized powder |
| mol wt | 29 kDa |
| Quality Level | 200  |
| specific activity | 0.2-0.6 units/mg protein (using benzylpenicillin) |
| storage temp. | 2-8°C |
| suitability | suitable for enzyme test |
| technique(s) | enzyme immunoassay: suitable |
| type | Type IV |
| Analysis Note: | Protein determined by biuret. |
| Application: | β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4524 is produced from Enterobacter cloacae and is provided as a lyophilized powder. |
| Application: | β-Lactamase from Enterobacter cloacae has been used in the maternal and fetal quantitative blood cultures to avoid any carryover phenomenon. It has also been used as a component in Mueller Hinton (MH) broth for placental cultures. |
| Biochem/physiol Actions: | β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring. |
| General description: | β-Lactamase is a monomeric enzyme of 29 kDa. This product is produced from Enterobacter cloacae and is provided as a lyophilized powder. It acts as a target protein for β-lactam antibiotics. This enzyme is present in Gram-negative bacteria. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units. |
| Packaging: | 100, 250 units in glass bottle |
| Physical form: | Lyophilized powder containing sodium phosphate buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.2-0.6 units/mg protein (using benzylpenicillin) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.2.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |