Phenol solution
SIGMA/P4557 - Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
Synonym: Tris-equilabrated Phenol
CAS Number: 108-95-2
Empirical Formula (Hill Notation): C6H6O
Molecular Weight: 94.11
EC Number: 203-632-7
MDL Number: MFCD00002143
Linear Formula: C6H6O
Product Type: Chemical
| assay | ≥99.0% (GC) |
| dilution | (for analytical testing) |
| form | liquid |
| grade | Molecular Biology |
| InChI | 1S/C6H6O/c7-6-4-2-1-3-5-6 |
| InChI key | ISWSIDIOOBJBQZ-UHFFFAOYSA |
| pH | 7.7-8.1 (25 °C) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | Oc1ccccc1 |
| storage temp. | 2-8°C |
| Application: | Phenol solution has been used: • as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate • for extraction of DNA from Arabidopsis thaliana Rosette leaves • in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol • as a component of extraction buffer to extract RNA from fruit pulp • for the extraction of protein from roots • for the isolation of fecal DNA • for the extraction of caged RNA |
| Biochem/physiol Actions: | The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol. |
| General description: | A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol. |
| Other Notes: | • 1 bottle Tris-equilabrated Phenol (product number P4557) • Equilibration Buffer (product B5658) |
| Packaging: | 100, 400 mL in glass bottle |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H311 + H331 - H314 - H341 - H373 - H411 |
| Precautionary statements | P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25-34-48/20/21/22-68 |
| Safety Statements | 24/25-26-28-36/37/39-45 |
| RIDADR | UN 2821 6.1 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (GC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352104 |