Pyruvate Oxidase from microorganisms
SIGMA/P4591 - lyophilized powder, ≥1.5 U/mg
Synonym: Pyruvate:oxygen oxidoreductase (phosphorylating)
CAS Number: 9001-96-1
EC Number: 232-644-5
MDL Number: MFCD00132148
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. pH Activity; 37 °C, in 50 mM K-phosphate buffer
Figure 3. Temperature activity; in 50 mM K-phosphate buffer, pH 5.7
Figure 4. pH Stability; 25 °C, 20-hr treatment with 50 mM buffer solution (containing 10 mM MgSO4, 10 μM FAD, 0.2 mM TPP): pH 4.0–6.0, acetate; pH 5.7–8.0 K-phosphate; pH 7.5–9.0, Tris-HCl
Figure 5. Thermal stability; 15-min. treatment with 50 mM K-phosphate buffer, 10 mM MgSO4, 10 μM FAD, 0.2 mM TPP, pH 6.0.
| form | lyophilized powder |
| mol wt | ~260 kDa |
| Quality Level | 200  |
| specific activity | ≥1.5 U/mg |
| storage temp. | −20°C |
| Application: | Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis. |
| Application: | Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate. |
| Biochem/physiol Actions: | Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles. |
| Biochem/physiol Actions: | Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine. |
| General description: | Isoelectric point : 4.3 Michaelis constant : 3.4 X 10-4M (Pyruvate) Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++ Optimum pH : 5.7 Optimum temperature : 65oC pH Stability : pH 5.7 - 6.5 (25oC, 20hr) Thermal stability : below 45oC (pH 6.0, 15min) |
| Other Notes: | One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37 °C. |
| Packaging: | 100 units in poly bottle |
| Physical form: | Lyophilized powder containing FAD and sugar as stabilizer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1.5 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.2.3.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |