Phosphorylase b from rabbit muscle
SIGMA/P4649 - For use as a marker in SDS-PAGE
Synonym: α-Glucan Phosphorylase; 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase; Glycogen Phosphorylase
CAS Number: 9012-69-5
EC Number: 232-737-0
MDL Number: MFCD00131905
Product Type: Chemical
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| form | powder |
| mol wt | observed mol wt ~97 kDa |
| packaging | vial of 0.5 mg |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase, and stress-70 protein. |
| Application: | Phosphorylase b from rabbit muscle is to be used as a marker in SDS-PAGE. Phosphorylase b is used during chemical cross-linking studies as a SDS-PAGE molecular weight standard. |
| General description: | Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles. Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state. The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.4.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |