Anti-PUMA/bbc3, N-Terminal antibody produced in rabbit
SIGMA/P4743 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Bcl-2 Binding Component 3; Anti-p53 Upregulated Modulator of Apoptosis
MDL Number: MFCD04040565
Product Type: Chemical
Immunoblotting
Western blot analysis of PUMA expression in human K562 cell lysate with Anti-PUMA (Cat. No. P4743) at 2 μg/mL.
Western blot analysis of PUMA expression in human K562 cell lysate with Anti-PUMA (Cat. No. P4743) at 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~23 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 2-4 μg/mL | |
| UniProt accession no. | Q96PG8  |
| Application: | Anti-PUMA/bbc3, N-Terminal antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | p53 upregulated modulator of apoptosis (PUMA) acts as a determinant of the levels of germ cells during ovarian development. It promotes apoptosis in various cellular stress insults. Deletion of the PUMA gene is observed in several kinds of human cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | p53 upregulated modulator of apoptosis (PUMA) protein is a member of the B-cell lymphoma 2 (BCL2) family. It is a pro-apoptotic BCL-2 homology 3 (BH3)-only protein and has a small BH3 domain. The PUMA gene is mapped on the human chromosome at 19q13.32. |
| Immunogen: | synthetic peptide corresponding to amino acids 2 to 16 of human PUMA-α. |
| Physical form: | Solution in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |