Anti-PADI4 (N-terminal) antibody produced in rabbit
SIGMA/P4749 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-PAD4; Anti-PADI5; Anti-PDI4; Anti-Peptidyl Arginine Deaminase Type IV; Anti-Peptidyl Arginine Deaminase V
MDL Number: MFCD09039202
Product Type: Chemical
Chromatin immunoprecipitation JOURNAL CITATION: Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. By: Zhang, X., Gamble, M. J., et al. in PLoS Genet, 2011. PubMed ID: 21655091 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1002112), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. By: Zhang, X., Gamble, M. J., et al. in PLoS Genet, 2011. PubMed ID: 21655091 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1002112), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Chromatin immunoprecipitation JOURNAL CITATION: Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. By: Zhang, X., Gamble, M. J., et al. in PLoS Genet, 2011. PubMed ID: 21655091 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1002112), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblot Analysis of PADI4 Lysates The extracts were separated on SDS-PAGE and blotted with Rabbit Anti-PADI4, IgG fraction of antiserum antibody (Cat. No. P4749), at a 1:1,000 dilution. The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminiscence substrate. Lanes 1. 293-T cells 2. 293-T cells transfected with PADI4 cDNA 3. HL60 cells 4. HL60 cells induced with 1.25% DMSO for three days
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PADI4 (N-terminal) antibody produced in Rabbit (Cat. No. P4749) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PADI4 (Lane 2) while no staining is detected in the wild type sample-HEK-293T (Lane 1). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 67 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells |
| microarray: suitable | |
| western blot (chemiluminescent): 1:500-1:1,000 using DMSO-treated HL60 cells | |
| UniProt accession no. | Q9UM07  |
| Application: | Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Application: | Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) |
| Biochem/physiol Actions: | PADs (also known as PADIs), peptidylarginine deiminases, are a family of enzymes, which convert protein-bound arginine residues to citrulline residues in the presence of calcium ions, a process termed deimination. Among the 5 isoforms of PADs, only PAD4 has been identified as an RA-susceptibility gene. |
| Biochem/physiol Actions: | Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PAD4 (also designated PADI4 or PAD type IV), one of the members of the PAD family. PAD4 was initially named PAD5. However, since it is closely related to rodent PAD4 it was renamed human PAD4. The most noticeable difference between the isotypes is their tissue-specific expression. Expression of the PAD4 gene is tightly linked to myeloid differentiation. It is expressed in granulocytes and monocytes and expressed in the nucleus. |
| General description: | Peptidyl arginine deiminase 4 (PADI4) is one of the four PADIs found in humans. The protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow. |
| Immunogen: | synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |