TWEEN® 80
SIGMA/P4780 - suitable for cell culture, suitable for insect cell culture, viscous liquid
Synonym: POE (20) sorbitan monooleate; Polyethylene glycol sorbitan monooleate; Polyoxyethylenesorbitan monooleate; Polysorbate 80
CAS Number: 9005-65-6
MDL Number: MFCD00082107
Product Type: Chemical
| application(s) | (ELISA and immunoassays) |
| CMC | 0.012 mM |
| composition | Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids) |
| density | 1.06 g/mL at 25 °C (lit.) |
| 1.07 g/cm3 at 25 °C (lit.) | |
| description | non-ionic |
| form | viscous liquid |
| InChI | 1S/C32H60O10/c1-2-3-4-5-6 |
| InChI key | RGPBUVUVZKQNHD-MDZDMXLPSA |
| mol wt | average mol wt ~1310 |
| Quality Level | 200  |
| SMILES string | CCCCCCCC/C=C/CCCCCCCC(=O) |
| technique(s) | cell culture | insect: suitable |
| cell culture | mammalian: suitable |
| Application: | Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines. |
| Application: | TWEEN® 80 has been used: • in Listeria monocytogenes enumeration using filtration method • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing |
| General description: | TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Packaging: | 1 gal in poly bottle |
| Packaging: | 100, 500 mL in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.06 g/mL at 25 °C (lit.); 1.07 g/cm3 at 25 °C (lit.) |
| UNSPSC | 12161700 |