Protease from Bacillus licheniformis
SIGMA/P4860 - ≥2.4 U/g
Synonym: Alcalase® 2.4L; Proteinase from Bacillus licheniformis; Subtilisin A
CAS Number: 9014-01-1
EC Number: 232-752-2
MDL Number: MFCD00132092
Product Type: Chemical
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| form | aqueous solution |
| Quality Level | 200  |
| specific activity | ≥2.4 U/g |
| storage temp. | 2-8°C |
| Analysis Note: | Activity expressed in Anson Units |
| Application: | Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. |
| Application: | The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides. It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities. |
| Biochem/physiol Actions: | Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains. |
| Biochem/physiol Actions: | Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat |
| General description: | Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases. |
| Legal Information: | A product of Novozyme Corp. |
| Legal Information: | Alcalase is a registered trademark of Novozymes Corp. |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H315 - H318 - H334 - H335 - H410 |
| Precautionary statements | P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-41-42-50 |
| Safety Statements | 22-24-26-36/37/39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥2.4 U/g |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.62 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |