Anti-PADI4 antibody produced in rabbit
SIGMA/P4874 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-PAD4; Anti-PADI5; Anti-PDI4; Anti-Peptidyl Arginine Deiminase Type IV; Anti-Peptidyl Arginine Deiminase V
MDL Number: MFCD09039201
Product Type: Chemical
Immunoblotting
Cell lysates of HL60 cells treated with DMSO (1.25% for three days) were separated on SDS-PAGE and blottied with Rabbit Anti-PADI4 (C-terminal) (Cat. No. P4874). The antibody was developed with Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. (Lane 1) Antibody 1:2,000 dilution; (Lane 2) Antibody 1:1,000 dilution; (Lane 3 Antibody 1:500 dilution).
Cell lysates of HL60 cells treated with DMSO (1.25% for three days) were separated on SDS-PAGE and blottied with Rabbit Anti-PADI4 (C-terminal) (Cat. No. P4874). The antibody was developed with Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. (Lane 1) Antibody 1:2,000 dilution; (Lane 2) Antibody 1:1,000 dilution; (Lane 3 Antibody 1:500 dilution).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 67 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells |
| western blot: 1:500-1:1,000 using DMSO-treated HL60 cells | |
| UniProt accession no. | Q9UM07  |
| Application: | Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting,immunoprec |
| Biochem/physiol Actions: | PAD4 is recruited to the pS2 promoter (an estrogen responsive gene) following hormone induction when the gene is transcriptionally downregulated. |
| Biochem/physiol Actions: | Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PAD4 (also designated PADI4 or PAD type IV), one of the members of the peptidyl arginine deiminases (PADIs). |
| General description: | Peptidyl arginine deiminase 4 (PADI4) protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow. |
| Immunogen: | synthetic peptide corresponding to amino acids 91-107 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human PADI4. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |