Phospholipase D from Streptomyces sp.
SIGMA/P4912 - Type VII, lyophilized powder, ≥150 units/mg solid
Synonym: Lecithinase D; Phosphatidylcholine phosphatidohydrolase
CAS Number: 9001-87-0
EC Number: 232-639-8
MDL Number: MFCD00131897
Product Type: Chemical
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| biological source | Streptomyces sp. |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥150 units/mg solid |
| storage temp. | −20°C |
| type | Type VII |
| Analysis Note: | Protein by biuret |
| Application: | Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies. |
| Application: | The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells. |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
| Biochem/physiol Actions: | Phospholipase D is glycerophospholipid-speci |
| Other Notes: | One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥150 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |