Polymyxin B sulfate salt

SIGMA/P4932 - powder, BioReagent, suitable for cell culture

Synonym: Polymixin; Polymixin B; Polymixin B sulfate

CAS Number: 1405-20-5
Empirical Formula (Hill Notation): C₅₅H₉₆N₁₆O₁₃ · 2H₂SO₄
Molecular Weight: 1385.61
EC Number: 215-774-7
MDL Number: MFCD00131991
Linear Formula: C₅₅H₉₆N₁₆O₁₃ · 2H₂SO₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P4932-1MU	1000000 units	$59.10 1/EA	Add To Favorites
45-P4932-5MU	5000000 units	$198.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	Gram-negative bacteria
form	powder
InChI	1S/C48H82N16O13.H2O4S/c1-27(2)24-37-47(76)59-32(11-19-52)41(70)56-31(10-18-51)43(72)61-35(14-22-65)39(68)54-21-13-34(45(74)57-33(12-20-53)44(73)64-38(48(77)63-37)25-28-6-4-3-5-7-28)60-42(71)30(9-17-50)58-46(75)36(15-23-66)62-40(69)29(8-16-49)55-26-67;1-5(2,3)4/h3-7,26-27,29-38,65-66H,8-25,49-53H2,1-2H3,(H,54,68)(H,55,67)(H,56,70)(H,57,74)(H,58,75)(H,59,76)(H,60,71)(H,61,72)(H,62,69)(H,63,77)(H,64,73);(H2,1,2,3,4)
InChI key	HNDFYNOVSOOGDU-UHFFFAOYSA-N
mode of action	cell membrane \| interferes
potency	≥6,000 USP units per mg
product line	BioReagent
Quality Level	200
SMILES string	O=C(C(NC(C(CCN)NC(CCCCC(C)CC)=O)=O)C(C)O)NC(CCN)C(NC(CCNC(C(NC(C(NC(C(CCN)N1)=O)CCN)=O)C(O)C)=O)C(NC(CCN)C(NC(CC2=CC=CC=C2)C(NC(CC(C)C)C1=O)=O)=O)=O)=O.O=S(O)(O)=O
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	Polymyxin B sulfate salt has been used: • for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor • as positive and negative control during microbicidal concentration (MCC) assay
Biochem/physiol Actions:	Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane. Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.
Biochem/physiol Actions:	Polymyxin B sulfate has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has been used in studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.
Disclaimer:	As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.
General description:	Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections. Chemical structure: peptide
Other Notes:	1mu,5mu
Other Notes:	Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.
Preparation Note:	Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Hazard Codes	Xn
Risk Statements	22
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352207

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