Anti-Peripherin antibody, Mouse monoclonal
SIGMA/P5117 - clone 8G2, purified from hybridoma cell culture
Synonym: Anti-NEF4; Anti-PRPH1
MDL Number: MFCD01633973
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Peripherin Clone:8G2 (Cat. No. P5117). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. PC12 2. Neuro-2a
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8G2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, bovine, pig, mouse, rabbit, feline, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using total cell extract from rat PC12 cells | |
| UniProt accession no. | P41219  |
| Application: | Anti-Peripherin antibody, Mouse monoclonal has been used in: • immunohistochemistry • western blotting • enzyme linked immunosorbent assay (ELISA) • immunofluorescence staining |
| Biochem/physiol Actions: | eripherin has been linked with axonal extension and regeneration of nerve fibers. It also has a role in neurite outgrowth propagation. Peripherin is a substrate of protein kinase B/Akt. Phosphorylation of this protein is induced after nerve injury. |
| Biochem/physiol Actions: | Peripherin is upregulated during pheochromocytoma PC12 cell differentiation. Later studies have detected peripherin also in certain central nervous system (CNS) neurons. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Peripherin (mouse IgG1 isotype) is derived from the 8G2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the full-length recombinant rat peripherin. Peripherin was originally identified as a neuronal intermediate filament protein-(IF) is thought to be expressed only in the peripheral nervous system (PNS). |
| General description: | Peripherin is an intermediate filament protein. It is expressed in peripheral and enteric neurons. The gene encoding this protein is localized on rat chromosome 7. |
| Immunogen: | full-length recombinant rat peripherin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |