Pepstatin A
SIGMA/P5318 - microbial, ≥90% (HPLC)
CAS Number: 26305-03-3
Empirical Formula (Hill Notation): C34H63N5O9
Molecular Weight: 685.89
EC Number: 247-600-0
MDL Number: MFCD00060740
Linear Formula: C34H63N5O9
Product Type: Chemical
| assay | ≥90% (HPLC) |
| biological source | microbial |
| form | powder |
| InChI | 1S/C34H63N5O9/c1-17(2)12- |
| InChI key | FAXGPCHRFPCXOO-LXTPJMTPSA |
| mp | 233 °C (dec.) (lit.) |
| Quality Level | 200  |
| SMILES string | CC(C)C[C@H](NC(=O)[C@H](C |
| solubility | ethanol: 1-2 mg/mL (with heat up to 60 °C) |
| storage temp. | 2-8°C |
| Application: | Can be used in conjunction with E64-d and Leupeptin A to inhibit the degradation of autophagic cargo inside autophagosomes. For this application, the working concentration is typically between 1-10 μM. |
| Biochem/physiol Actions: | Pepstatin A is an inhibitor of acid proteases (aspartyl peptidases). It forms a 1:1 complex with proteases such as pepsin, renin, cathepsin D, bovine chymosin, and protease B (Aspergillus niger). The inhibitor is highly selective and does not inhibit thiol proteases, neutral proteases, or serine proteases. Solubilized γ-secretase and retroviral protease are also inhibited by Pepstatin A. It has been used to characterize proteases from several sources. |
| Biochem/physiol Actions: | Potent inhibitor of acid proteases, including pepsin, renin and cathepsin D and many microbial aspartic proteases. Effective concentration 1 μM. Stable for about 1 day. |
| General description: | Pepstatin A is a naturally occurring chemotactic peptide and inhibitor of aspartic proteases that was initially isolated from culture filtrates of various Actinomycetes species, with the initial name simply of “pepstatin”. This name was later modified to “pepstatin A” to distinguish the original pepstatin from later derivatives. Pepstatin A notably contains the unusual amino acid 4-amino-hydroxy-6-methylh |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 5, 25 mg in glass bottle |
| Preparation Note: | Pepstatin can be dissolved at 1 mg/ml in 10% (v/v) acetic acid in methanol (9:1 methanol:acetic acid). The inclusion of acetic acid may be necessary to dissolve this peptide in methanol or DMSO. It has been dissolved at 1 to 2 mg/ml in ethanol, but heat may be required for complete dissolution. Solutions of Pepstatin A can be heated as high as 60 °C without any decomposition of the peptide. Stock solutions at 1 mg/ml are stable at least a week at 4 °C. A 1 mM solution in methanol or DMSO is stable for months at -20 °C. If solutions become darker yellow, the reagent is hydrolyzing. A working concentration of 1 μM is stable for at least one day at room temperature. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| mp | 233 °C (dec.) (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |