Anti-Podoplanin antibody produced in rabbit
SIGMA/P5374 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Aggrus; Anti-GP36; Anti-Glycoprotein, 36-KD; Anti-OTS8; Anti-T1α gene; Anti-T1A; Anti-T1A2
MDL Number: MFCD09265367
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 20 μg/mL Anti-Podoplanin antibody produced in Rabbit (Cat. No. P5374), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin fixed, paraffin-embedded Human skin were stained with 20 μg/mL Anti-Podoplanin antibody produced in Rabbit (Cat. No. P5374), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mLExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of 293-T cells were separated on SDS-PAGE and probed with Anti-Podoplanin antibody produced in Rabbit (Cat. No. P5374). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 10 μg/mL antibody 2. 20 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~38 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using heat retrieved, human skin sections |
| western blot (chemiluminescent): 5-10 μg/mL using human HEK-293 or rat NRK cells | |
| UniProt accession no. | Q86YL7  |
| Application: | Anti-Podoplanin antibody produced in rabbit has been used in immunoblotting. |
| Application: | Anti-Podoplanin antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Podoplanin (PDPN) is expressed in many cancers. In cancerous cells, it induces platelet aggregation and epithelial-mesenchymal transition. The protein is an immunohistochemical marker of lymphatic vessels. Podoplanin also has a role in embryonic development. |
| Biochem/physiol Actions: | Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It is detectable in human angiosarcomas, lymphangiomas and in Kaposi′s sarcomas. Experiments involving depletion of T1a/podoplanin in mice indicate a critical regulatory role for it in formation of the lymphatic vasculature. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Podoplanin (PDPN) is a transmembrane sialoglycoprotein. It consists of 162 amino acids and is part of the type-1 transmembrane sialomucin-like glycoprotein family. The protein is expressed in various tissues. The PDPN gene is localized on human chromosome 1p36.21. |
| General description: | Podoplanin is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. Podoplanin, also named E11 antigen, is present in normal, inflammatory and neoplastic lymphatic capillary endothelium. It is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells. |
| Immunogen: | synthetic peptide corresponding to amino acids 24-40 of human podoplanin with an N-terminal added cysteine, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide and 1% bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |