Protease from Bacillus licheniformis
SIGMA/P5380 - Type VIII, lyophilized powder, 7-15 units/mg solid
Synonym: Proteinase from Bacillus licheniformis; Subtilisin A; Proteinase from Bacillus licheniformis; Subtilisin A
CAS Number: 9014-01-1
EC Number: 232-752-2
MDL Number: MFCD00132092
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5380-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5380-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5380-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5380-250MG
| biological source | Bacillus licheniformis |
| foreign activity | DNase ≤5.0 Kunitz units/mg solid |
| RNase ≤0.05 Kunitz units/mg solid | |
| form | lyophilized powder |
| mol wt | 27 kDa |
| purified by | crystallization |
| Quality Level | 200  |
| solubility | 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear |
| H2O: soluble, colorless | |
| specific activity | 7-15 units/mg solid |
| storage temp. | −20°C |
| type | Type VIII |
| Application: | Non-specific Protease Activity Assay Video |
| Application: | The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. |
| Application: | This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. |
| Biochem/physiol Actions: | Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions. |
| Biochem/physiol Actions: | Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat |
| General description: | Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa. |
| General description: | The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 250 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H334 - H335 - H410 |
| Precautionary statements | P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-41-42-50 |
| Safety Statements | 22-24-26-36/37/39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 1 |
| activity | specific activity: 7-15 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.62 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |