Phosphoglucose Isomerase from baker’s yeast (S. cerevisiae)
SIGMA/P5381 - Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonym: D-Glucose-6-phosphate ketol-isomerase; PGI; Phosphosaccharomutase
CAS Number: 9001-41-6
EC Number: 232-603-1
MDL Number: MFCD00131887
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5381-50KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5381-5KU
| biological source | bakers yeast |
| color | white |
| concentration | ≥3 mg/mL protein Biuret |
| form | ammonium sulfate suspension |
| mol wt | tetramer 119,500 Da±600 |
| Protein ID accession no. | NP_009755.1   |
| Quality Level | 200 |
| specific activity | ≥400 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| type | Type III |
| UniProt accession no. | P12709 |
| Application: | Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:• to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels. • as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity. • to convert fructose to glucose while evaluating fructose content in kudzu roots. |
| Biochem/physiol Actions: | Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator. |
| General description: | Research area: Cell Signaling Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical. |
| Other Notes: | One unit will convert 1.0 μmole of |
| Physical form: | Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥400 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 5.3.1.9 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |