Pectinesterase from orange peel
SIGMA/P5400 - lyophilized powder, ≥150 units/mg protein
Synonym: Pectin methylesterase; Pectin pectylhydrolase
CAS Number: 9025-98-3
EC Number: 232-807-0
MDL Number: MFCD00131812
Product Type: Chemical
| composition | Protein, 20-50% biuret |
| form | lyophilized powder |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | ≥150 units/mg protein |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| Analysis Note: | Protein determined by biuret. |
| Application: | Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene. |
| Application: | The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes. |
| Biochem/physiol Actions: | Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research . |
| Other Notes: | One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1000 units in glass bottle |
| Physical form: | Contains (NH4)2SO4 and sodium chloride |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥150 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |