o-Phenylenediamine
SIGMA/P5412 - tablet, 20 mg substrate per tablet
Synonym: 1,2-Diaminobenzene; 1,2-Phenylenediamine; OPD
CAS Number: 95-54-5
Empirical Formula (Hill Notation): C6H8N2
Molecular Weight: 108.14
EC Number: 202-430-6
MDL Number: MFCD00007721
Linear Formula: C6H8N2
Product Type: Chemical
o-Phenylenediamine (OPD) is a chromogenic substrate suitable for use in ELISA procedures which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3 M H2SO4, and read at 492 nm.
| bp | 256-258 °C |
| fluorescence | λex 337 nm; λem 417 nm (after derivatization) |
| form | tablet |
| InChI | 1S/C6H8N2/c7-5-3-1-2-4-6( |
| InChI key | GEYOCULIXLDCMW-UHFFFAOYSA |
| mp | 100-102 °C |
| Quality Level | 200  |
| SMILES string | Nc1ccccc1N |
| storage temp. | 2-8°C |
| vapor density | 3.7 (vs air) |
| vapor pressure | 0.01 mmHg ( 25 °C) |
| Application: | o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm. |
| Application: | o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation. |
| Preparation Note: | Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets. |
| Symbol |    GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 - H317 - H319 - H341 - H351 - H410 |
| Precautionary statements | P202 - P273 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | T,N |
| Risk Statements | 20/21-25-36-40-43-50/53-68 |
| Safety Statements | 28-36/37-45-60-61 |
| RIDADR | UN 1673 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 276.8 °F - closed cup |
| Flash Point(C) | 136 °C - closed cup |
| bp | 256-258 °C |
| mp | 100-102 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |