Protease from Bacillus licheniformis
SIGMA/P5459 - glycerol solution (50%)
Synonym: Proteinase from Bacillus licheniformis; Subtilisin A; Proteinase from Bacillus licheniformis; Subtilisin A
CAS Number: 9014-01-1
MDL Number: MFCD00132092
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | Bacillus licheniformis |
| concentration | ≥50 mg/mL |
| form | glycerol solution (50%) |
| Quality Level | 200  |
| solubility | 0.85% sodium chloride: soluble, clear (Diluted) |
| H2O: miscible | |
| specific activity | ≥7 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens. |
| Application: | Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle. |
| Biochem/physiol Actions: | Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat |
| General description: | The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated. |
| Packaging: | 5 g in glass bottle |
| Packaging: | Package size based on protein content. |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H318 - H334 - H412 |
| Precautionary statements | P273 - P280 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-37/38-41-42-50 |
| Safety Statements | 23-26-39-45-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥7 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.62 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |