Phosphate buffered saline
SIGMA/P5493 - 10× concentrate, BioPerformance Certified, suitable for cell culture
Synonym: PBS
MDL Number: MFCD00131855
Product Type: Chemical
| application(s) | advanced drug delivery |
| cation traces | heavy metals (as Pb): ≤5 ppm |
| color | colorless |
| foreign activity | DNAse, none detected |
| NICKase, none detected | |
| Protease, none detected | |
| RNAse, none detected | |
| form | solution |
| grade | BioPerformance Certified |
| Molecular Biology | |
| impurities | Bioburden, tested |
| endotoxin, tested | |
| ≤5 ppm heavy metals (as Pb) | |
| InChI | InChI=1S/2ClH.2K.3Na.2H3O |
| InChI key | LOKCTEFSRHRXRJ-UHFFFAOYSA |
| pH | 7.2-7.6 (25 °C) |
| Quality Level | 100 ![]() |
200 ![]() |
|
| SMILES string | OP(=O)(O)[O-].OP(=O)([O-] |
| solubility | water: soluble |
| sterility | 0.2 μm filtered |
| storage condition | (Tightly closed) |
| suitability | suitable for |
| suitable for flow cytometry | |
| suitable for microscopy | |
| technique(s) | cell culture | mammalian: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable |
| Application: | Phosphate buffered saline has been used: • as a cell wash solution in human dental pulp stem cells (DPSCs) • for perfusion studies in dissected brain samples • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling • as wash solution for ovary and sperm during in vitro culture and fertilization |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4. |
| Packaging: | 4L package size comes in a box with a spigot for spill free dispensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |

