Protein Kinase A from bovine heart
SIGMA/P5511 - ≥0.8 units/μg protein, lyophilized powder
Synonym: Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
CAS Number: 9026-43-1
MDL Number: MFCD00132121
Product Type: Chemical
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| composition | Protein, ≥70% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥0.8 units/μg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00517  |
| P05131 |
| Analysis Note: | Protein determined by biuret. |
| Application: | Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases. |
| Application: | Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin. |
| Biochem/physiol Actions: | Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins. |
| Biochem/physiol Actions: | Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain. |
| General description: | Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme. |
| Other Notes: | One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP. |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | 10 mg in poly bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5. |
| Preparation Note: | Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.8 units/μg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.37 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |