Phosphoglucose Isomerase from Bacillus stearothermophilus
SIGMA/P5538 - lyophilized powder, 300-1,000 units/mg protein
Synonym: D-Glucose-6-phosphate ketol-isomerase; PGI; Phosphosaccharomutase
CAS Number: 9001-41-6
EC Number: 232-603-1
MDL Number: MFCD00131887
Product Type: Chemical
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| composition | Protein, ≥60% biuret |
| form | lyophilized powder |
| mol wt | 189 kDa |
| Quality Level | 200  |
| specific activity | 300-1,000 units/mg protein |
| storage temp. | −20°C |
| Application: | Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus. |
| Application: | The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti. |
| Biochem/physiol Actions: | Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator. |
| General description: | The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2. |
| Other Notes: | One unit will convert 1.0 μmole of |
| Physical form: | lyophilized powder containing Tris buffer |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 300-1,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 5.3.1.9 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |