Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

SIGMA/P5542 - lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym: 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming; PI-PLC

CAS Number: 37288-19-0
EC Number: 232-638-2
MDL Number: MFCD00131896
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P5542-5UN	5 units	$305.00 1/EA	Add To Favorites
45-P5542-25UN	25 units	$1040.00 1/EA	Add To Favorites

Figure 10. General structure of GPI anchored proteins. The lipid tails of the phosphatidylinositol moiety are shown inserted through the outer cell membrane and held by the lipid bilayer.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5542-25UN

Properties
Descriptions
Safety Info.
Basic Data

foreign activity	Phopholipase C (lecithinase) ≤1 units/mg protein
	Sphingomyelinase ≤40 units/mg protein
form	lyophilized powder
Quality Level	300
specific activity	≥1,000 units/mg protein (Lowry)
storage temp.	−20°C

Analysis Note:	Acetylcholinesterase is measured according to Ellman, et al.
Application:	Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
Biochem/physiol Actions:	Used for the release of GPI anchored proteins from the membrane.
General description:	Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Other Notes:	One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Packaging:	5, 25 units in glass bottle
Physical form:	Contains phosphate buffer salts, EDTA and stabilizer

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥1,000 units/mg protein (Lowry)
Storage Temp.	−20°C
Enzyme Commission (EC) Number	4.6.1.13 ( BRENDA \| IUBMB )
UNSPSC	12352204

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