Anti-Pen-2 antibody produced in rabbit
SIGMA/P5622 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD07370427
Product Type: Chemical
Immunoblotting
Whole cell extracts of 293 cells transfected with human Pen-2 (Lanes A–C) were separated on SDS-PAGE, blotted with Rabbit Anti-Pen-2 (Cat. No. P5622) at a 1:1,000-1:2,000 dilution, and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) using a chemiluminescent substrate. Specificity of antibody is confirmed by inhibition of staining with the Pen-2 immunizing peptide (20 μg/mL, Lane C).
Whole cell extracts of 293 cells transfected with human Pen-2 (Lanes A–C) were separated on SDS-PAGE, blotted with Rabbit Anti-Pen-2 (Cat. No. P5622) at a 1:1,000-1:2,000 dilution, and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) using a chemiluminescent substrate. Specificity of antibody is confirmed by inhibition of staining with the Pen-2 immunizing peptide (20 μg/mL, Lane C).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 10 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 3-6 μg/mL using whole extract of human kidney 293 cells expressing human Pen-2 | |
| UniProt accession no. | P49810  |
| Application: | Anti-Pen-2 antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Anti-PEN2 antibody recognizes Pen-2 (10 kDa). |
| Biochem/physiol Actions: | Pen-2 is a critical component of the presenilin (PS)1/γ-secretase and PS2/γsecretase complexes. siRNA interference mediated down regulation of pen-2 is due to reduced PS levels, impaired nicastrin (Nct) maturation and deficient γ secretase complex formation. Pen-2 preferentially interacts with PS1 and induce PS1 endoproteolysis. In addition, it also stabilizes PS fragment heterodimer within the γ-secretase complex subsequent to PS endoproteolysis. PEN-2 plays a vital role in γ-secretase complex maturation in the absence of PS endoproteolysis. Mutation in the PEN-2 gene leads to the development of familial comedones syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Presenilin enhancer γ-secretase subunit (Pen-2/ PSENEN) gene, with four exons spanning 1.4 kb, is mapped to human chromosome 19q13.12. Pen-2 is an integral membrane protein with 101 amino acids. The protein is characterized with two transmembrane domains and a cytosolic loop domain, with the C- and N-termini facing the lumen of the endoplasmic reticulum. |
| Immunogen: | synthetic peptide encoding amino acids 86-101 located at the C-terminus of human pen-2, conjugated to KLH. This sequence is identical in mouse pen-2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |