Anti-Phosphoserine antibody, Mouse Monoclonal
SIGMA/P5747 - clone PSR-45, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Phosphoserine; Phospho Ser; Phospho serine; Phospho−Ser; Phospho−serine
Product Type: Chemical
Western Blotting JOURNAL CITATION: Interaction of connexin43 and protein kinase C-delta during FGF2 signaling. By: Niger, C., Hebert, C., et al. in BMC Biochem, 2010. PubMed ID: 20338032 Image collected and cropped by CiteAb from the following publication, (http://www.biomedcentral.com/1471-2091/11/14), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-Phosphoserine antibody produced in Mouse, Clone: PSR-45 (Cat. No. P5747). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody 3. Negative Control
ELISA Monoclonal Anti-Phosphoserine antibody produced in Mouse, Clone: PSR-45 (Cat. No. P5747), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304), was serially diluted to detect Phosphoserine by indirect ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PSR-45, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA | |
| microarray: suitable | |
| western blot: 2.5-5.0 μg/mL using total rat brain extract. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | The antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting. |
| Biochem/physiol Actions: | By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site. |
| Biochem/physiol Actions: | Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |