Anti-p53 antibody, Mouse monoclonal
SIGMA/P5813 - clone BP53-12, ascites fluid
Synonym: Anti-TRP53; Anti-
MDL Number: MFCD00164709
Product Type: Chemical
Immunohistochemistry
Methacarn-fixed, paraffin-embedded section of human breast carcinoma stained with Monoclonal Anti-p53, clone BP53-12 (Cat. No. P5813) and ExtrAvidin® Peroxidase Staining Kit (Cat. No. EXTRA2).
Methacarn-fixed, paraffin-embedded section of human breast carcinoma stained with Monoclonal Anti-p53, clone BP53-12 (Cat. No. P5813) and ExtrAvidin® Peroxidase Staining Kit (Cat. No. EXTRA2).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BP53-12, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 53 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein | |
| UniProt accession no. | P04637  |
| Application: | Monoclonal Anti-p53 antibody produced in mouse has been used in: • western blotting • immunohistochemistry • immunocytochemistry • enzyme linked immunosorbent assay (ELISA) |
| Application: | Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines. |
| Biochem/physiol Actions: | The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells. |
| Immunogen: | recombinant human wild-type p53 protein. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |