Poly-L-lysine hydrobromide
SIGMA/P5899 - mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Synonym: PLL HBr
CAS Number: 25988-63-0
MDL Number: MFCD00237230
Product Type: Chemical
| biological source | synthetic (organic) |
| form | lyophilized powder |
| impurities | <10% water (by Karl Fischer) |
| InChI | 1S/C18H38N6O4/c19-10-4-1- |
| InChI key | WBSCNDJQPKSPII-KKUMJFAQSA |
| mol wt | ≥300,000 |
| packaging | pkg of 5 mg |
| product line | BioXtra |
| Quality Level | 200  |
| shipped in | ambient |
| SMILES string | Cl.NCCCCC(N)C(O)=O |
| solubility | H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow |
| sterility | γ-irradiated |
| storage temp. | −20°C |
| surface coverage | 4 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | Molecular weight based on viscosity. Also assayed by MALLS. |
| Application: | Poly-L-lysine hydrobromide has been used to coat:• 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation • flat bottom 96-well plates for in vitro neuronal stem cell differentiation • T75 flasks for primary astrocytic cultures |
| Biochem/physiol Actions: | Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs. |
| Disclaimer: | Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C. |
| Other Notes: | For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource . |
| Other Notes: | Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |