Phosphatase, Alkaline from Escherichia coli
SIGMA/P5931 - lyophilized powder, 30-60 units/mg protein (in glycine buffer)
Synonym: Orthophosphoric-
CAS Number: 9001-78-9
EC Number: 232-631-4
MDL Number: MFCD00131849
Product Type: Chemical
Unit Definition One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Approximately 3 DEA units equal one Glycine unit.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5931-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5931-200UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P5931-500UN
| composition | Protein, ~50% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 30-60 units/mg protein (in glycine buffer) |
| storage temp. | −20°C |
| Application: | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P5931 has been used during immunoblots to treat cell membranes prior to Tau1 incubation. |
| Application: | The enzyme from Sigma has been used to develop a synthetic nanopore membrane. This membrane mimics protein channels that are regulated by phosphorylation/dephospho |
| Biochem/physiol Actions: | Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| Biochem/physiol Actions: | The enzyme is a phosphohydrolase having an optimal pH of 10 in vitro. The actual optimum pH varies depending on the nature and concentration of the substrate, the type of buffer, the phosphate acceptor, and to some extent the nature of the isoenzymes. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. The activity is inhibited by 1,10-phenanthroline monohydrate, diethylenetriaminepentaac |
| General description: | Alkaline phosphatase (ALP) of E.coli is a member of an enzyme group that possesses intragenic complementation. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C. |
| Packaging: | 100, 200, 500 units in glass bottle |
| Physical form: | Lyophilized powder containing Tris buffer salts, MgSO4, and ZnSO4 |
| Preparation Note: | Chromatographically purified |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 30-60 units/mg protein (in glycine buffer) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |