Pectolyase from Aspergillus japonicus
SIGMA/P5936 - suitable for plant cell culture, lyophilized powder
Synonym: PEL1
MDL Number: MFCD00131813
Product Type: Chemical
| application(s) | agriculture |
| form | lyophilized powder |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | ≥0.3 unit/mg solid |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| technique(s) | cell culture | plant: suitable |
| Application: | Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments. |
| Application: | Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls. |
| Application: | Used in plant protoplast preparation to digest cell wall prior to organelle isolation. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research . |
| Other Notes: | One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C. |
| Other Notes: | Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 100, 250 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.3 unit/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.15 ( BRENDA  | IUBMB ) |
| UNSPSC | 10171502 |