Phosphatase substrate
SIGMA/P5994 - 40 mg tablets
Synonym: 4-Nitrophenyl phosphate disodium salt hexahydrate; pNPP disodium salt hexahydrate
CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.
| concentration | 36-44 mg/tablet |
| form | tablet |
| impurities | p-nitrophenol, essentially free |
| InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7 |
| InChI key | KAKKHKRHCKCAGH-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | O.O.O.O.O.O.[Na+].[Na+].[ |
| solubility | H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.) |
| storage temp. | −20°C |
| Application: | Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm. |
| Application: | Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm. |
| Application: | Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC). |
| Application: | The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins. |
| General description: | Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kin |
| Other Notes: | Substrate for the determination of acid and alkaline phosphatases. |
| Packaging: | 25 tabs in glass bottle |
| Preparation Note: | Contains 40 mg substrate per tablet |
| Preparation Note: | Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |