Anti-Platelet-Derived Growth Factor antibody produced in goat
SIGMA/P6101 - IgG fraction of antiserum, lyophilized powder
Synonym: Anti-PDGF
MDL Number: MFCD00165456
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| neutralization: suitable | |
| western blot: suitable | |
| UniProt accession no. | P01127  |
| P04085 |
| Application: | Anti-Human Platelet-Derived Growth Factor (PDGF) antibody may be used for ELISA at a working concentration of 0.25 μg/ml. The antibody has been used for ELISA at a dilution of 1:500 using human serum.. For immunoblotting, a concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions and has been used to neutralize PDGF of primary Human dermal fibroblasts (HDFs) and human platelet-enriched plasma. |
| Biochem/physiol Actions: | The antibody will neutralize the biological activity of natural human PDGF, recombinant human PDGF-AB, PDGF-AA and PDGF-BB and natural porcine PDGF-BB. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Platelet-derived growth factor (PDGF) is a serum growth factor purified from human platelets. The members of PDGF are homodimers of A-, B-, C- and –D polypeptide chains and an AB heterodimer. PDGF is a mitogen and stimulates the proliferation of cells of connective tissue and glial cells, survival, differentiation, chemotaxis and wound healing. The cellular effects of PDGF isoforms are mediated by binding to α- and β-receptors which leads to activation of downstream signaling via PI3K, STAT and Ras/MAPK. Overexpression of PDGF isoforms has been observed in various malignancies including gliomas, osetosarcoma, chronic myelomonocytic leukemia and non-small-cell lung cancers Anti-Human Platelet-Derived Growth Factor (PDGF) specifically recognizes natural human PDGF. It will also neutralize the activity of recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. It shows cross reactivity with recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. |
| Immunogen: | natural human PDGF. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) with 5% trehalose. |
| Preparation Note: | Purified using protein G. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |