Pyroglutamate Aminopeptidase from Pyrococcus furiosus
SIGMA/P6236 - recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein
Synonym: L-Pyrrolidone carboxyl peptidase
EC Number: 232-981-8
MDL Number: MFCD00132139
Product Type: Chemical
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| assay | ~90% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | 24.072 kDa by amino acid sequence |
| 28 kDa by SDS-PAGE | |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥5.0 units/mg protein |
| storage temp. | −20°C |
| Application: | Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides. |
| Application: | The enzyme from Sigma has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin. |
| Application: | Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. |
| Biochem/physiol Actions: | Pyroglutamate Aminopeptidase (PGP 1) interacts with immunoglobulin, functions as inflammatory cytokine and modulates immune response. The levels PGP 1 is elevated during inflammation. |
| Biochem/physiol Actions: | This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100 °C and the optimal pH range is 6.0 to 9.0. |
| General description: | Pyroglutamate Aminopeptidase from Pyrococcus furiosus, also called the deblocking aminopeptidase, is a 42 kDa protein and belongs to aminopeptidase A family. It shares sequence homology with aminopeptidase in the active site, with conserved zinc and cobalt binding residues. |
| Other Notes: | One unit will hydrolyze 1 μmol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37 °C. |
| Physical form: | Lyophilized powder containing sodium phosphate |
| Preparation Note: | Reconstitute the vial of enzyme with 50 μl of 50 mM sodium phosphate, pH 7.0, with 10 mM DTT and 1 mM EDTA. The reconstituted solution should be stored at -20 °C. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~90% (SDS-PAGE) |
| activity | specific activity: ≥5.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.19.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |