Protein Tyrosine Phosphatase 1B human
SIGMA/P6244 - recombinant, expressed in E. coli
Synonym: PTP-1B
MDL Number: MFCD01635664
Product Type: Chemical
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| concentration | ≥0.5 mg/mL |
| form | solution |
| mol wt | 37.4 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| relevant disease(s) | diabetes; obesity; cancer |
| shipped in | dry ice |
| specific activity | ≥20 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P18031  |
| Application: | Protein Tyrosine Phosphatase 1B (PTP1B) human has been used in treating cell lysate prior to immunoprecipitation and protein pull-down studies. It has also been used in PTP1B activity assay and also for the evaluation of inhibitors like betulinic acid (BA) and sodium orthovanadate (Na3VO4), and 6-chloro-3-formyl-7-methy |
| Application: | Protein tyrosine phosphatase 1B (PTP1B) is a pharmaceutical target used to study the treatment of various diseases such as obesity, cancer and type II diabetes mellitus. |
| Application: | The inhibitory effect of 8-(4-chlorophenylthio)-cA |
| Biochem/physiol Actions: | Polymorphism in the gene PTPN1 is associated with type 2 diabetes and obesity. |
| Biochem/physiol Actions: | PTP-1B is a recombinant, non-transmembrane protein expressed in E. coli containing amino acid residues 1-322 of human PTP-1B. It has a molecular mass of 37.4 kDa. It is an abundant intracellular enzyme that is thought to act as a negative regulator of certain signaling pathways. It dephosphorylates tyrosine-phosphorylated proteins and peptides, and is usually localized in the cytostolic domain of the ER. |
| Biochem/physiol Actions: | PTP1B dephosphorylates c-Src in several human breast cancer cell lines has a regulatory role for PTP1B in the control of c-Src kinase activity. PTP1B is regulates the cardiovascular effects of leptin. PTP1B controls the JAK/STAT signaling pathway by dephosphorylation of JAK2. |
| General description: | Protein tyrosine phosphatase 1B human (PTP1B) is encoded by the gene PTPN1. In human chromosome, the gene PTPN1 is localized on 20q13.13. The protein has 37 kDa catalytic domain and the C-terminal hydrophobic residues targets the protein to the cytoplasmic side of the endoplasmic reticulum membrane. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of a phosphopeptide, EGFR fragment 988-998, per minute at pH 7.2 at 30 °C |
| Physical form: | Supplied as a solution in 50 mM HEPES (pH 7.2), 1 mM EDTA, 5 mM DTT, and 0.05% NP-40 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |