Anti-Parkin antibody, Mouse monoclonal
SIGMA/P6248 - clone PRK8, purified from hybridoma cell culture
Synonym: Anti-AR-JP; Anti-LPRS2; Anti-PARK2; Anti-PDJ
MDL Number: MFCD02263896
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Parkin Clone: PRK8 (Cat. No. P6248). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Rat Brain 2. Mouse Brain
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PRK8, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, hamster, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract |
| Application: | Monoclonal anti-parkin antibody has been used in: • Enzyme linked immunosorbent assay (ELISA) • Immunoblotting • Immunoprecipitation[63} • Immunocytochemistry • to label parkin in pull-down assay |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons. |
| General description: | Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human. |
| Immunogen: | recombinant human Parkin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |