p53 human
SIGMA/P6374 - recombinant, expressed in baculovirus infected Sf21 cells
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| application(s) | genomic analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | aqueous solution |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf21 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | dot blot: suitable |
| UniProt accession no. | P04637  |
| Application: | p53 human has been used in dot blot. |
| Application: | Useful for the study of postranslational modification of p53, or protein-protein interaction studies. |
| Biochem/physiol Actions: | In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53. |
| Biochem/physiol Actions: | p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer. |
| Biochem/physiol Actions: | p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome. |
| General description: | Histidine-tagged, full length human p53 |
| General description: | p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains. The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1. |
| Physical form: | Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA, and 1 mM DTT. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |