Poly-L-lysine hydrobromide
SIGMA/P6516 - mol wt 4,000-15,000 by viscosity
Synonym: L-Lysine homopolymer hydrobromide
CAS Number: 25988-63-0
MDL Number: MFCD00237230
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6516-100MG
This image depicts SKU: P6516-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6516-1G
This image depicts SKU: P6516-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6516-500MG
This image depicts SKU: P6516-500MG
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
12 Principles of Green Chemistry: Principle 5—Safer Solvents and Auxiliaries. This product utilizes reduced volumes of auxiliary substances that are less hazardous.
12 Principles of Green Chemistry: Principle 8—Unnecessary derivatization (use of blocking groups, protection/ deprotection, temporary modification of physical/chemical processes) should be minimized or avoided if possible, because such steps require additional reagents and can generate waste.
| application(s) | cell analysis |
| color | white to off-white |
| form | solid |
| greener alternative category |  , Re-engineered |
| greener alternative product characteristics | Designing Safer Chemicals Safer Solvents and Auxiliaries Reduce Derivatives Learn more about the Principles of Green Chemistry . |
| greener alternative product score | old score: 51 new score: 43 Find out more about DOZN™ Scoring |
| InChI | 1S/C18H38N6O4/c19-10-4-1- |
| InChI key | WBSCNDJQPKSPII-KKUMJFAQSA |
| mol wt | ≤15,000 by MALLS |
| 4,000-15,000 by viscosity | |
| Quality Level | 200 |
| SMILES string | Cl.NCCCCC(N)C(O)=O |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Poly- • as a component of polyplexes and in DNA condensation experiments • for coating circular mica disks for atomic force microscopy studies and SuperFrost glass slides for immunofluorescence studies of in HL-60 cells • as a reference standard for generating calibration curve for the quantification of ε- Poly- |
| Biochem/physiol Actions: | Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor. |
| Disclaimer: | Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Designing Safer Chemicals”, "Safer Solvents and Auxiliaries" and “Reduce Derivatives”. Click here to view its DOZN scorecard. |
| Other Notes: | Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 25, 100, 500 mg in poly bottle |
| Preparation Note: | This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352209 |