Phospholipase A2 from porcine pancreas
SIGMA/P6534 - ammonium sulfate suspension, ≥600 units/mg protein
Synonym: Lecithinase A; PLA2; Phosphatidylcholine 2-acylhydrolase
CAS Number: 9001-84-7
EC Number: 232-637-7
MDL Number: MFCD00131890
Product Type: Chemical
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| form | ammonium sulfate suspension |
| Quality Level | 200  |
| specific activity | ≥600 units/mg protein |
| storage temp. | 2-8°C |
| UniProt accession no. | P00592  |
| Analysis Note: | Protein determined by biuret. |
| Application: | Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation. |
| Biochem/physiol Actions: | Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells. |
| Biochem/physiol Actions: | It has a high catalytic activity on aggregated substrates compared to monomeric substrates. |
| General description: | Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of soybean |
| Packaging: | 1, 5, 10 mg in glass bottle |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 solution, pH 5.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥600 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |