Phospholipase C from Bacillus cereus
SIGMA/P6621 - ≥200 units/mg protein
Synonym: Lecithinase C; Lipophosphodiesterase I; PC-PLC; Phosphatidylcholine cholinephosphohydrolase
CAS Number: 9001-86-9
MDL Number: MFCD00131895
Product Type: Chemical
Degumming of feedstock oils by hydrolyisis of phospholipids can be catalyzed by phospholipase C. Treatment of
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6621-250UN
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| form | lyophilized powder |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 23-27 kDa by SDS-PAGE |
| Quality Level | 300 |
| solubility | H2O: soluble 1.0 mg/mL, faintly hazy to hazy |
| specific activity | ≥200 units/mg protein |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| Application: | Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling. It is used to degrade inositol-containing phospholipids. Phospholipase C from Bacillus, product P6621, has been used to study how flhF affects the export of PLC and other secretory virulence factors. |
| Application: | The enzyme from Sigma has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms. |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. |
| Biochem/physiol Actions: | Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 μmole of water soluble organic phosphorus from |
| Packaging: | 250 units in glass bottle |
| Physical form: | Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |