Anti-Phosphothreonine antibody, Mouse monoclonal
SIGMA/P6623 - clone PTR-8, purified from hybridoma cell culture
Synonym: Monoclonal Anti-
Product Type: Chemical
Western Blotting Whole extract of A431 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Phosphothreonine antibody produced in Mouse, Clone: PTR-8 (Cat. No. P6623). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 10 μg/mL antibody 2. Negative Control
ELISA Monoclonal Anti-Phosphothreonine antibody produced in Mouse, Clone: PTR-8 (Cat. No. P6623), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304), was serially diluted to detect Phosphothreonine by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PTR-8, monoclonal |
| concentration | ~2.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: 0.5-1 μg/mL | |
| microarray: suitable | |
| western blot: 5-10 μg/mL using A431 cell extracts |
| Application: | Monoclonal Anti-Phosphothreonine antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA), dot blot, immunoprecipitation, immunocytochemistry and immunoblotting. It has also been used in Akt kinase assay to detect phosphorylation status on serine and threonine residue in the wild type or mutant type δ-catenin. |
| Biochem/physiol Actions: | Monoclonal Anti-Phosphothreonine reacts with phosphorylated threonine both as a free amino acid or when conjugated to carriers such as BSA or KLH, using ELISA and dot blot. It does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP or ATP. |
| Biochem/physiol Actions: | Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue. Various activation processes are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-Tyr/p-Ser/p-Thr). Many different mitogenic systems, such as the epidermal growth factor (EGF), platelet derived growth factor (PDGF) and insulin receptor systems contain Tyr/Ser/Thr kinase domains that autophosphorylate specific Tyr/Ser/Thr residues upon binding of their ligands. T cell antigen receptor complex or receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated Tyr/Ser/Thr. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containi |
| General description: | Monoclonal Anti-Phosphothreonine (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with phosphothreonine conjugated to keyhole limpet hemocyanin (KLH). |
| General description: | Mouse monoclonal clone PTR-8 anti-Phosphothreonine antibody reacts with phosphorylated threonine both as a free amino acid or when conjugated to carriers such as BSA or KLH, using ELISA and dot blot. It does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP or ATP. |
| Immunogen: | phosphothreonine conjugated to keyhole limpet hemocyanin (KLH). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |