Phosphorylase b from rabbit muscle
SIGMA/P6635 - lyophilized powder, ≥20 units/mg protein, 2× crystallization
Synonym: α-Glucan Phosphorylase; 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase; Glycogen Phosphorylase
CAS Number: 9012-69-5
EC Number: 232-737-0
MDL Number: MFCD00131905
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6635-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6635-25MG
| biological source | rabbit muscle |
| foreign activity | phosphoglucomutase ≤1.0% |
| phosphorylase a ≤10% | |
| phosphorylase kinase ≤0.5% | |
| phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1% | |
| form | lyophilized powder |
| impurities | ~0.01 μmol/mg protein 5′-AMP (This low level will not interfere with phosphorylase and phosphorylase kinase assays.) |
| mol wt | 97,200 Da by calculation |
| purified by | 2× crystallization |
| Quality Level | 300  |
| specific activity | ≥20 units/mg protein |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place) |
| storage temp. | −20°C |
| technique(s) | mass spectrometry (MS): suitable |
| UniProt accession no. | A0A5F9CLZ9  |
| A0A5F9CRR0 |
|
| A0A5F9D1C4 |
|
| A0A5F9DLL9 |
| Application: | Phosphorylase b from rabbit muscle has been used: • in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits • in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in the charge-reducing buffer • for the preparation of p32 labeled phosphorylase A using phosphorylase kinase and [32P]ATP • in phosphorylase phosphatase assay • in enzyme assay as a positive control to ensure the reaction system for the activity determination was adopted |
| Biochem/physiol Actions: | Phosphorylase b is a non-active form and is present in resting muscles. Phosphorylase b kinase activity increases significantly when the Mg2+:ATP ratio exceeds 1. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate. Glycogen phosphorylase (PG) enzyme plays a vital role in the first step of glycogenolysis. In the initial stage of glycogenolysis, glycogen phosphorylase(GP) breaks α-1,4- -glycosidic bonds, releasing the glucose-1-phosphate (G1P)molecule. When incubated with the proper concentrations of glucose-1-phosphate without the addition of primer, rabbit muscle phosphorylase B was found to be capable of forming protein-bound alpha-1,4 glucosyl chains. |
| General description: | Research area: Cell Signaling Glycogen phosphorylase (PG), a specialized complex allosteric enzyme has an evolutionarily conserved gene sequence. GP contains a family of three isozymes such as muscle GP (mGP), liver GP (lGP), and brain GP (bGP) in humans. |
| Other Notes: | One unit will form 1.0 μmole of α- |
| Packaging: | 10, 25, 100 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing lactose, 5′-AMP, and Mg(OAc)2 (10 μmole per 100 mg protein) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |